The more, the merrier.Ĭhoose the download for your operating system below, extract the archive to a new folder and run it.
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They provide molecular weight standards to estimate the size of proteins separated by gel electrophoresis like SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Protein ladders or molecular weight markers are among the most commonly used reagents in biochemistry experiments. coli and affinity protein purification, and to research laboratories desiring positive controls for recombinant protein expression and purification. These Penn State Protein Ladder expression plasmids also constitute useful reagents for teaching laboratories to demonstrate recombinant expression in E. 50 ml of culture is sufficient to produce 20,000 lanes of individual ladder protein or 3750 lanes of each set of coexpressed ladder proteins. For more efficient production, we have created two polycistronic expression vectors which coexpress the 10, 30, 50, 100 kD proteins or the 20, 40, 60, 80 kD proteins. We have also constructed plasmids to express 150 and 250 kD proteins. Each protein migrates appropriately on SDS-PAGE gels, is expressed at very high levels (10–50 mg per liter of culture), is easy to purify via histidine tags and can be detected directly on Western blots via engineered immunoglobulin binding domains. The system includes plasmids which express 10, 15, 20, 30, 40, 50, 60, 80 and 100 kD proteins in E. We have created the Penn State Protein Ladder system to produce protein molecular weight markers easily and inexpensively (less than a penny a lane). The solution it suggests for people having problems when using the ALSA sound system is to use OSS instead. For the answer to this problem, I turned to the Doom 3 FAQ, since Quake 4 is built on the Doom 3 engine, and the FAQ said it would not repeat suggestions/problems outlined there. Afterward, I could start the game as a normal user. I fixed the problem by going into /usr/local/games/quake4/q4base as root and entering chmod 755 *.pk4. On those three, only root had any permissions at all user and group were not even allowed read permission. I noticed while copying files off the CDs that the permissions are different on the first CD than they are on the others. But in my case, I found had a couple of issues to resolve before proceeding.įirst, I found that I could run Quake4 only as root. If all goes well, it takes only a couple of seconds to finish the install from that point. Make sure the paths and options are as you want them, then click on Begin Install. At this point, you’ll see the installer window. The Punkbuster license agreement is next, you can agree or not as you like, but if you don’t agree, you won’t be able to play on a lot of Quake 4 servers. You’ll have to agree to the Quake 4 license agreement to continue. Then, still as root, change to that directory and enter: Depending on what the mount point is for your CD - /cdrom works on mine - the following command should do it.Ĭp /cdrom/Setup/Data/q4base/*.pk4 /usr/local/games/quake4/q4baseĪfter you’ve copied all the files, copy the installer to /usr/local/games/quake4. You’ll need to insert each of the four CDs and - as root - copy the needed files to your hard drive. Due to issues with Breezy and the Audigy 2, I am using the 5.1 channel AC97 2.3-compliant sound card on the mainboard.īefore you copy the needed files from the retail CDs prior to running the installer, create the directories needed for Quake 4 by executing the following command as root:Įach of the four CDs contains a directory named /Setup/Data/q4base. It has a PNY Verto G-Force 6600 with 256MB of memory sitting in a PCI Express slot and an SB Live! Audigy 2 sound card. I installed Quake 4 on my homebrew AMD64 Sempron 3000+ CPU with 512MB memory, sitting on an MSI K8N mainboard, running Ubuntu Breezy. You can’t turn around and ignore the ads, as that same window is where eventually you see a “Click here to begin download” message. I eventually had success at 3Ddownloads, which puts in a queue and shows ads as you wait your turn. At some sites, you can get a free download after clicking past a seemingly endless number of pages designed to do nothing but ring up their ad sales, and then find the free download is throttled all the way down and allows only two simultaneous free downloads - but they will gladly sell you a download. Of course, there are other sites to get it from. Every time I tried I got a “421 Too many users – please try again later” response. I found it impossible to download from id Software. Getting the Linux installer was a little frustrating. You must be running version 2.2.4 or later glibc, and a kernel version of at least the 2.4 level is recommended. Hardware requirements include a 2GHz or better processor with 512MB of memory, an ATI Radeon or Nvidia GeForce video card, and a sound card supported by OSS or ALSA. You’ll also need to download the Linux installer - quake4-linux-.12.x86.run - from id Software or a mirror site. The product FAQ explains that in addition to the CD key contained in the retail package - required to play the game - you’ll need to copy a bunch of files from the CDs as part of the installation process. But that’s not enough to let you play Quake 4 on Linux. The box contained 4 CDs, a game guide, and a license key. As soon as I read the news on Slashdot that id Software‘s just released Quake 4 was already available as both a Linux client and game server, I ran out and dropped $50 for the game. Just a stone’s throw from the Gouffre de Padirac in the Lot, are the Grottes de Presque, offering yet another cathedral of wonders. Lifts are available for those who do not wish to tackle the long staircase back to the surface. Stretching 94 metres high, this extraordinary space is a feast for the eyes: in the words of Édouard-Alfred Martel, the man who discovered the Gouffre de Padirac, “nowhere else has nature built more extraordinary monuments”. Clinging on to the roof by a slender thread, the ‘Grand Pendeloque’ is a 60-metre colossal stalactite, its image reflected in the shimmering ‘Lac de Pluie’ (the Lake of Rain).Īfter disembarking the boat and proceeding on foot, the cave opens out into the Salle du Grand Dôme, and the ascending walkways allow visitors ample opportunity to marvel at stunning crystal accumulations. King of caves, this one really does have it all, and is one of the most-visited cave complexes in France.ĭescending 103 metres down a steep staircase, visitors are ushered into a boat that transports them along a subterranean river, with staggering views of the formations that trickle from the canopy. The Gouffre de Padirac’s standing as one of the best caves in the Lot, and indeed France, means it’s a standard feature on any keen explorer’s French itinerary. The 45-minute tour provides an excellent introduction to the history and geology of the cave, allowing visitors plenty of time to explore this magnificent cavern. For those willing to pay a little extra, it’s even possible to be lowered from the roof of the chamber in a specially designed, rotating viewing platform, offering close-up views of the stalactites and petrified waterfalls that descend from the canopy.Īs the sound and light show begins, the story of the cave is revealed, stage-by-stage, casting eerie shadows and illuminating the remarkable crystal formations that hang from the walls. Today, visitors enter this Dordogne cave in darkness. In winter, the hole at the top of this extraordinary cavern emits plumes of smoke, which, long ago, led local residents to believe that the fires of hell were burning just below the surface, ready to ensnare unsuspecting sinners. Once at the bottom, you can’t help but look up in wonder at the small circle of sky that now looks decidedly faraway.Known locally for centuries as the ‘Devil’s Hole’, the Gouffre de Proumeyssac is the stuff of legends. This enormous sink hole then leads into a cave system, created by millennia of subterranean underground water erosion which has carved out spectacular caverns in the limestone, full of enormous stalactites and stalagmites deep under the ground.įirst, you descend by lift or the impressive metal staircase, the design of which was inspired by the Eiffel Tower of Paris. In times gone by it was often referred to by the local population as the devil’s hole, and it’s a sight to behold as you and other awestruck visitors gaze down into the enormous 75-metre chasm into the earth, 33 meters wide. More than 24 million visitors (and counting) have come to marvel at this natural wonder, and it continues to enthrall those who see it. Discovered by the famous French cave explorer Edouard Martel, it was opened to the public in 1899. The Gouffre de Padirac is one of the largest chasms in Europe. 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It is going to be what it is going to be, and whether VV made the changes to pander, or this is their artistic vision (aside: I had 2 people tell me they always thought the Assassin was Asian in D2…they never really thought about it enough to look further than a 1st impression), here we are. I am quite sure if there is a real majority out there who share your feelings, and show their displeasure with their wallets, they’ll either change, or, they’ll refuse to change and you can feel justified in feeling like the change was made to pander.įinally, and this isn’t directed at anyone personally, it really is a moot point, I think. Superman is an alien to Earth, and his skin color therefore is really open to interpretation.Īs far as what you want or don’t want…if it bothers you that much, get a refund, and tell them why. Bruce Wayne is American, and therefore can be of any heritage. A good analogy would be if you had a character from Sweden, making that character black would be much less realistic. Well, Black Panther is a special case a hero born in an African country where everyone is black…kind of hard to flip that. So they DID listen to the D3 feedback that cartoon type chars are not something that is good for the Diablo series. They also emphasize the dark and gritty for D4 too. That was huge for a long time and stuck in the minds of a lot of people. I don’t care for the amazon face, but I really don’t think it was political and I DO think it was based on the whole “D2 was perfect in part because it was so dark and realistic and gritty - D3 was too bright and cartoony”. So they go through the old concept art, fan art, game images, cultural depictions of amazons, feedback on D2 (dark and gritty which caused backlash for D3 being cartoony). It was inspired by old things sure - but the current artists will use some of their own interpretations and vision hence the 70/30 thing we were told up front. EVERYTHING had to be re-imagined in much more detail in the High def version. Well, they could just stick with the original pixels? That is unrealistic though. If anything she was a woman that people respected for being a bad*ss. Some of those characters are not pretty per social norms at all and yet changing any of them would cause backlash just as changing Amazon is causing backlash.Īmazon has never been a barbie doll character or a WAIFU. It does not take a genius to understand that change to characters in a game that defined a genre will cause backlash.Īnd as explained before, these female characters in WoW have fans from all walks of life, there’s no single demographic: While there are certainly some people at extremes as MissC mentions, when the community as a whole, just talks openly and honestly about the character appearances they’ve been playing with for decades, many people just aren’t happy with change.Ĭhange with many people is like an OCD thing - it’s not good. Along with men, you will see women and some in the LGBTQIA+ community being upset about changing models. Go watch LLamma’s VODs discussing D2R Amazon and character models. That’s absurd and you’ve made a staggeringly cliche assumption about the D2 playerbase. This is why I have trouble understanding why Blizzard actually allows these threads. 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Further the changes in interface are also obvious the shadows drops are still there but the glassy glimpse for the textures is gone now. The split screen view, the better performance in terms of consistency, more support for touchpad, Wi-Fi and Bluetooth are some of the features that are to be considered while reviewing the OS. Mac OS X Yosemite 10.10 is experienced to have more ease in terms of system management and tool bars settings. After giving our MacBook a new look, some newly added and innovative features were there like, improved gaming processing, enhanced visuals and some other interface related improvements were also noticed. If it’s about an experience the noticeable part is, where one upgrades from Mavericks to Yosemite 10.10 it does slow downs your Mac (A few friends have complaint), whether or not that happened to you, but at least in our case it is not the scenario, the up-gradation went quite smoothly, freely and hassle free. The reasons are definite and are commendable, as performance based improvements and a brand new look, stayed as the topic of praise. The free upgrade was available for the customers and is considered as one amongst the Apple’s prime products launched so far. Pros and Cons are existent for the product but the iOS inspired interface, the browsing improvements experienced via Safari, improved iOS integration, the newly added icons and enchanted mail features are some striking points where the OS has an expertise. Since Apple has launched it’s 10th release for Mac OS X Series, named as Mac OS X Yosemite, but there were mixed reviews about this revamped hit by the developer’s. Mac OS X 10.10 Yosemite: Product’s Review: Give a new refreshing look to your Mac and MacBooks interface with Mac OS X Yosemite. Download Mac OS X 10.10 Yosemite Latest Installer for free. |
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